When Clover, Alex and Sam's mothers discover that their daughters are spies, they completely freak out and inform Jerry that they are putting an end to it immediately. The spies go back to regular civilian life. Meanwhile, Mandy, Dominique and Caitlin all become affected by an evil shampoo from one of the spies' missions (the girls accidentally dropped it into Mandy's pool). Mandy and her cronies become evil “spy-sassins” (anti-spy agents) and kidnap the spies' mothers. Their plan? To sequester them in hopes that they can capture the spies when they try to save their moms. Sam, Clover and Alex realize that their own carelessness is what got their mothers in trouble, so they become spies again and ultimately save their moms. But in doing so, they get captured by Mandy! Now with the spies trapped back at The Groove, Mandy turns them into spy-sassins so that the Sam, Clover and Alex will destroy one another. Luckily for the girls, Jerry trained their mothers to be spies, and the moms swoop in a save the girls from each other. Now with the spies back to normal (thanks to a serum created by Jerry), the mothers and daughters round up evil Mandy and her cronies! In the end, the mothers allow their daughters to remain WOOHP agents.
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